Prothrombin Gene Mutation Test | Austin Pathology

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Prothrombin Gene Mutation

Alternate Names	FII, Factor II, G20210A, PGM, PTG
Ordering Information	Prothrombin (Factor II) and Factor V Leiden mutation detection will now be performed by GeneXpert Factor II and Factor V Assay and will be tested together. To order, use the Molecular Haematology Request form (pricing included): Molecular Haematology Test Request Form V11.
Laboratory	Molecular Diagnostics - Genetics
Specimen	Blood
Container	EDTA (purple)
Additional Collection Instructions	The specimen must be received in Austin Pathology within 24 hrs of collection. Please collect a DEDICATED EDTA tube unless Factor V Leiden is also requested. Prothrombin Gene Mutation and/or Factor V Leiden testing can be performed on the same 1 x 4mL EDTA tube. A citrate tube is not acceptable.
Minimum Adult Volume	1mL
Minimum Paediatric Volume	None
Frequency	Several times per week

These PDF documents can be downloaded for your reference

Order of Draw (PDF)

Swab Collection Guide (PDF)

Pre-Transfusion Specimen Requirements (PDF)

Requirements for Pathology Service (PDF)

Test Code	APTG
Container ID	EW
Storage Instructions	Store at 4°C
Additional (Storage) Instructions	Instructions for: Metropolitan & Regional Specimen Receptions Whole blood samples should be refrigerated upon processing to ensure sample stability. Refrigerated samples are stable for 15 days. Frozen (between -20°C and -80°C) samples are stable for up to 2 months. Frozen whole blood samples should not be thawed/frozen more than once. Instructions for: Heidelberg Specimen Reception Store whole blood samples in the FII & FV bucket in the walk-in fridge.
Transport Instructions	Transport at 4°C
Additional Transport Instruction	Instructions for: Regional Specimen Receptions All whole blood samples should be transported to the Heidelberg Laboratory at 4°C.
Laboratory Notes	Instructions for: Regional and Metropolitan Specimen Receptions If this test is requested with Factor V Leiden, both tests do not need to be coded. APTG and AFVL will be added together.