Austin Pathology

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Flow Cytometry

Alternate Names
Cell Surface Markers, CLL, Immunophenotype, Leukaemia Panel, Leukaemia Screen, Lymphoma, Lymphoproliferative Screen, NHL
Ordering Information

REQUEST FLOW CYTOMETRY ON TISSUE FOR:

  Suspected lymphoma (past history of lymphoma, lymphadenopathy, radiology/imaging suggests lymphoma)

    Flow is of no value in Hodgkin lymphomas

 

TISSUE SAMPLE REQUIREMENTS

  Separate fresh tissue specimen in sterile pot or in tissue preservative (RPMI, pink liquid – preferred if sending after hours)

  Label as urgent

  If uncertain, please discuss with Anatomical Pathologist

 

PLEASE NOTE: Anatomical pathology cannot/will not divide smaller specimens such as core biopsies, these should have separate specimens for flow cytometry and histology.

We will only divide large specimens >1-2 cubic cm (e.g. whole lymph nodes, spleen)

For non lymph node tissue specimens that are small (often small and very important) ideally cases should be discussed with a pathologist before or during a procedure to ascertain the most appropriate distribution of tissue. An Anatomical Pathologist is available by telephone at all times.

Histology should usually be prioritized as this is more likely to be diagnostic.

 

Laboratory
Haematology
Specimen
See collection instructions
Container
See collection instructions
Additional Collection Instructions

Blood:  5 mL blood LITHIUM HEPARIN tube no gel, PLUS 4 mL blood EDTA tube.  Please do not combine with other tests using the same sample type, as separate tubes must be collected for these tests.

Bone Marrow:  LITHIUM HEPARIN or EDTA tube.  Separate tubes must be collected for the tests. Arrange with the Haematology Registrar, ph: 9496 5981.

Lymph Node:  Sterile container (no fixative) and send immediately to Anatomical Pathology.

Body Fluid:  Send immediately to Anatomical Pathology.

Minimum Adult Volume
None
Minimum Paediatric Volume
None
Notes

On Fridays and prior to public holidays, specimens must be received in Pathology before 3pm and marked as URGENT.

Frequency
Monday - Friday
Reference Interval
  > 18 yrs 16-18 yrs 12-16yrs 10-12 yrs 6-10 yrs 5-6yrs
WCC X 109/L 4.0-11.0 4.0-11.0 4.0-11.0 4.0-11.0 4.0-11.0 5.0-15.0
Lymph cells/µL 1000-4000 1000-4000 1200-5200 1200-5200 1200-5200 1500-7000
CD3% 65-83 56-84 56-84 60-76 60-76 56-75
CD4% 35-59 31-52 31-52 31-47 31-47 28-47
CD8% 15-35 18-35 18-35 18-35 18-35 16-30
CD16&56% 5-20 3-22 3-22 4-17 4-17 4-17
CD3 cells/µL 1090-3020 1000-2200 1000-2200 1200-2600 1200-2600 1400-3700
CD19 cells/µL 190-550 110-570 110-570 270-860 270-860 390-1400
CD4 cells/µL 650-2000 530-1300 530-1300 650-1500 650-1500 700-2200
CD8 cells/µL 330-1310 330-920 330-920 370-1100 370-1100 490-1300
CD16 cells/µL 130-540 70-480 70-480 100-480 100-480 130-720
T4:T8 1.1-4.0 1.1-4.0 0.7-2.6 0.7-2.6 0.9-3.5 0.9-3.5

 

  4-5 yrs 3-4 yrs 2-3 yrs 1-2 yrs 6 mo - 1 yr 3-6 mths  < 3mths
WCC x 109/L 5.0-15.0 5.0-16.5 5.0-16.5 5.0-16.5 5.0-18.0 5.0-18.0 5.0-18.0
Lymph cells/µL 1500-7000 2000-8000 2000-8000 3000-9000 2800-9100 2800-9100 2800-9100
CD3% 56-75 56-75 56-75 53-75 49-76 51-77 53-84
CD19% 14-33 14-33 14-33 16-35 14-37 11-41 6-32
CD4% 28-47 28-47 28-47 32-51 31-56 35-56 35-64
CD8% 16-30 16-30 16-30 14-30 12-24 12-23 12-28
CD16&56% 4-17 4-17 4-17 3-15 3-15 3-14 4-18
CD3 cells/µL 1400-3700 1400-3700 1400-3700 2100-6200 1900-5900 2500-5600 2500-5600
CD19 cells/µL 390-1400 390-1400 390-1400 720-2600 610-2600 430-3000 430-3000
CD4 cells/µL 700-2200 700-2200 700-2200 1300-3400 1400-4300 1800-4000 1800-4000
CD8 cells/µL 490-1300 490-1300 490-1300 620-2000 500-1700 590-1600 590-1600
CD16 cells/µL 130-720 130-720 130-720 180-920 160-950 170-830 170-830
T4:T8 1.2-3.5 1.2-3.5 1.0-3.9 1.1-3.6 1.1-3.6 1.5-3.8 1.5-3.8