Cell Surface Markers, CLL, Immunophenotype, Leukaemia Panel, Leukaemia Screen, Lymphoma, Lymphoproliferative Screen, NHL, Mantle Cell Lymphoma, Myeloma, Lymphoproliferative Screen Body Fluid, Lymphoproliferative Screen Tissue

FLOW

Flow Cytometry

None

REQUEST FLOW CYTOMETRY ON TISSUE FOR:

• Suspected lymphoma (past history of lymphoma, lymphadenopathy, radiology/imaging suggests lymphoma)

Flow cytometry is not diagnostic for Hodgkin's lymphoma.

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PLEASE NOTE: Anatomical pathology cannot/will not divide smaller specimens such as core biopsies, these should have separate specimens for flow cytometry and histology.

The laboratory will only divide large specimens >1 - 2 cubic cm (e.g. whole lymph nodes, spleen)

For non lymph node tissue specimens that are small (often small and very important), cases should ideally be discussed with a pathologist before or during a procedure to ascertain the most appropriate distribution of tissue. 

Contact the Anatomical Pathology Laboratory on: 03 9496 5285 or 03 9496 5543 to speak with a pathologist.

Histology should usually be prioritized as this is more likely to be diagnostic.

Instructions for: Metropolitan Collection Centres

• DO NOT COLLECT AFTER MIDDAY FRIDAY OR AT ALL ON SATURDAY.
• DO NOT COLLECT AFTER MIDDAY THE DAY BEFORE A PUBLIC HOLIDAY.

Instructions for: Regional Collection Centres

• DO NOT COLLECT ON FRIDAY OR SATURDAY.
• DO NOT COLLECT THE DAY BEFORE A PUBLIC HOLIDAY.
  

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BLOOD

• DEDICATED 5 mL blood LITHIUM HEPARIN tube no gel, AND 4 mL blood EDTA tube.

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BONE MARROW (BMFLOW)

• LITHIUM HEPARIN or EDTA tube.  Separate tubes must be collected for the tests. Arrange with the Haematology Registrar, ph: 03 9496 5981.

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LYMPH NODE

• Sterile container (no fixative) and send immediately to Anatomical Pathology.

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BODY FLUID

• If only Flow Cytometry is requested, send immediately to Flow Cytometry.
• If Flow Cytometry and Histology/Cytology are requested, send immediately to Anatomical Pathology

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TISSUE 

• Separate fresh tissue specimen in sterile pot or in tissue preservative (RPMI, pink liquid – preferred if sending after hours)
• Label as urgent
• If uncertain, please discuss with Anatomical Pathologist

Transport ambient at room temperature

Store ambient at room temperature

Monday - Friday

2mL

Add ons for this test cannot be performed.

If retrospective flow cytometry is required, please discuss with the flow cytometry laboratory.