Austin Pathology

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Flow Cytometry

Alternate Names
Cell Surface Markers, CLL, Immunophenotype, Leukaemia Panel, Leukaemia Screen, Lymphoma, Lymphoproliferative Screen, NHL, Mantle Cell Lymphoma
Ordering Information

REQUEST FLOW CYTOMETRY ON TISSUE FOR:

  Suspected lymphoma (past history of lymphoma, lymphadenopathy, radiology/imaging suggests lymphoma)

    Flow is of no value in Hodgkin lymphomas

 

TISSUE SAMPLE REQUIREMENTS

  Separate fresh tissue specimen in sterile pot or in tissue preservative (RPMI, pink liquid – preferred if sending after hours)

  Label as urgent

  If uncertain, please discuss with Anatomical Pathologist

 

PLEASE NOTE: Anatomical pathology cannot/will not divide smaller specimens such as core biopsies, these should have separate specimens for flow cytometry and histology.

We will only divide large specimens >1-2 cubic cm (e.g. whole lymph nodes, spleen)

For non lymph node tissue specimens that are small (often small and very important) ideally cases should be discussed with a pathologist before or during a procedure to ascertain the most appropriate distribution of tissue. An Anatomical Pathologist is available by telephone at all times.

Histology should usually be prioritized as this is more likely to be diagnostic.

 

Laboratory
Flow Cytometry
Specimen
See collection instructions
Container
See collection instructions
Additional Collection Instructions

Blood:  DEDICATED 5 mL blood LITHIUM HEPARIN tube no gel, PLUS 4 mL blood EDTA tube.  Please do not combine with other tests using the same sample type, as separate tubes must be collected for these tests.

Bone Marrow:  LITHIUM HEPARIN or EDTA tube.  Separate tubes must be collected for the tests. Arrange with the Haematology Registrar, ph: 9496 5981.

Lymph Node:  Sterile container (no fixative) and send immediately to Anatomical Pathology.

Body Fluid:  Send immediately to Anatomical Pathology.

 

Instructions for: Metro Collection Centres

DO NOT COLLECT AFTER MIDDAY FRIDAY OR AT ALL ON SATURDAY.

 

Instructions for: Regional Collection Centres

 DO NOT COLLECT ON FRIDAY OR SATURDAY.

Minimum Adult Volume
None
Minimum Paediatric Volume
None
Notes

On Fridays and prior to public holidays, specimens must be received in Pathology before 3pm and marked as URGENT.

Frequency
Monday - Friday
Test Code
Refer to laboratory notes
Storage Instructions
Store specimen at room temperature
Transport Instructions
Transport at room temperature
Additional Transport Instruction

Instructions for: Regional Specimen Receptions

DO NOT SPIN. KEEP AT ROOM TEMPERATURE. Forward sample to the Heidelberg Laboratory, Flow Cytometry.

 

Instructions for: Heidelberg Specimen Reception

DO NOT SPIN. KEEP AT ROOM TEMPERATURE.

If a sample is collected outside of the specified collection timeframe, forward the sample to Flow Cytometry.

Laboratory Notes

 

FLOW SUBS PNH PRE34 HLAB27 MISCSO
NHL:
  • Lymphoma Panel
  • Lymphoproliferative Panel
  • Lymphocyte Cell Surface Markers (CSM) - GVH
  • Lymphocyte Marker Studies
  • Lymphocyte Immunophenotyping

Lymphocyte Subsets:

  • CD4 Count
  • CD4 / CD8 Count
  • T-cells
  • B-cells

Paroxysmal Nocturnal Hemoglobinuria:

  • CD59
CD34 (Urgent) Human Leukocyte Antigen B27 PNH FLAER (Send to Peter Mac)
Myeloma BAL        
Leukemia          
Any Bone Marrow          
Any Tissue          
Any CSF