Donath Landsteiner Test Test | Austin Pathology

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Donath Landsteiner Test

Alternate Names	PCH, Paroxysmal Cold Haemoglobinuria
Ordering Information	This test can only be performed at the following hospital-associated collection centres: Austin Hospital Heidelberg Repatriation Mercy Hospital Mildura Shepparton Swan Hill Echuca
Laboratory	Blood Bank
Specimen	Blood
Container	9ml PLAIN (red top)
Additional Collection Instructions	LABORATORY BASED COLLECTION ONLY. This test requires 2 x 9mL PLAIN (red top) tubes - please ensure tubes are filled completely This test must be collected using a HOT BOX kit, maintained at 37°C (kit stocked with pre-warmed syringes, collection tubes, etc.). Collection materials should only be taken out immediately prior to use. After blood collection, the specimen tubes should be immediately returned to the HOT BOX and transported promptly to the laboratory. Contact your lab or hospital associated collection centre for a HOT BOX.
Minimum Adult Volume	6mL
Minimum Paediatric Volume	None
Frequency	As required

These PDF documents can be downloaded for your reference

Order of Draw (PDF)

Swab Collection Guide (PDF)

Pre-Transfusion Specimen Requirements (PDF)

Requirements for Pathology Service (PDF)

Test Code	DL
Container ID	SB, EB, S
Opt. Specimen	None
Storage Instructions	Store at 4°C
Additional (Storage) Instructions	Instructions for: Metropolitan and Regional Specimen Receptions Store whole blood before testing at 37°C. If centrifuged & separated, aliquots should be stored at 4°C.
Transport Instructions	Transport at 4°C
Additional Transport Instruction	Instructions for: Regional Specimen Receptions Send the primary tube (cells) and separated serum at 4°C to the Heidelberg Laboratory for testing.
Laboratory Notes	Instructions for: Regional Laboratories The primary sample (PLAIN (red top)) must be maintained at 37°C until clot has completed formed and retracted within tube. Separate serum from clot with a pipette in a secondary aliquot tube. Centrifuge the secondary aliquot tube to remove trace red cells and then transfer the serum to a tertiary aliquot tube which is to be labelled, dated and signed and stored at 4°C until testing is performed. Instructions for: Heidelberg Specimen Reception For samples collected at Austin: remove samples from the HOT BOX and immediately place into a 37°C incubator. Blood Bank staff will collect the samples from the incubator and process specimens. Samples are to remain in their original collection tubes. For samples arriving from Regional labs: Forward samples to blood bank.