Donath Landsteiner Test (Blood) Test | Austin Pathology

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Donath Landsteiner Test (Blood)

Alternate Names	PCH, Paroxysmal Cold Haemoglobinuria, Donath Landsteiner Ab
Test Code	DL
Testing Laboratory	Transfusion (Blood Bank)
Specimen Type	Blood
Container Type	9 mL PLAIN (Red Top)
Medicare Rebate	Yes
Out of Pocket Costs	None
Ordering Information	This test can only be performed at the following hospital-associated collection centres: Austin Hospital Heidelberg Repatriation Mercy Hospital Mildura Shepparton Swan Hill Echuca
Collection Instructions	LABORATORY BASED COLLECTION ONLY. This test requires 2 x 9mL PLAIN (red top) tubes - please ensure tubes are filled completely This test must be collected using a HOT BOX kit, maintained at 37°C (kit stocked with pre-warmed syringes, collection tubes, etc.). Collection materials should only be taken out immediately prior to use. After blood collection, the specimen tubes should be immediately returned to the HOT BOX and transported promptly to the laboratory. Contact your laboratory or hospital associated collection centre for a HOT BOX.
Transport Instructions	Transport at 37°C using Hot Box kit Send to the laboratory as soon as possible
Storage Instructions	None
Testing Frequency	As Required
Min Test Volume	6mL
Add On Test Suitability	Add ons for this test cannot be performed.

Container ID	SB, EB, S
CSR Instructions	Instructions for: Metropolitan & Regional Specimen Receptions Store whole blood before testing at 37°C. If centrifuged & separated, aliquots should be stored at 4°C. Instructions for: Regional Specimen Receptions Send the primary tube (cells) and separated serum at 4°C to the Heidelberg Laboratory for testing.
Laboratory Instructions	Instructions for: Regional Laboratories The primary sample (PLAIN (red top)) must be maintained at 37°C until clot has completed formed and retracted within tube. Separate serum from clot with a pipette in a secondary aliquot tube. Centrifuge the secondary aliquot tube to remove trace red cells and then transfer the serum to a tertiary aliquot tube which is to be labelled, dated and signed and stored at 4°C until testing is performed. Instructions for: Heidelberg Specimen Reception For samples collected at Austin: remove samples from the HOT BOX and immediately place into a 37°C incubator. Blood Bank staff will collect the samples from the incubator and process specimens. Samples are to remain in their original collection tubes. For samples arriving from Regional laboratories: Forward samples to blood bank.
Accredited Test	Yes

These PDF documents can be downloaded for your reference

Order of Draw (PDF)

Swab Collection Guide (PDF)

Pre-Transfusion Specimen Requirements (PDF)

Requirements for Pathology Service (PDF)