Heparin Induced Thrombocytopenia Confirmation Assay (Blood) Test

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Heparin Induced Thrombocytopenia Confirmation Assay (Blood)

Alternate Names

HIT Confirmation Assay, HITEIA, HIT ELISA

Test Code

HITEIA

Testing Laboratory

Referred Laboratory

Specimen Type

Blood

Container Type

Sodium Citrate (Light Blue Top)

Medicare Rebate

Yes

Out of Pocket Costs

None

Ordering Information

As of 29 September 2026, this test is no longer performed on-site due to assay availability with the manufacturer. HIT Screen Positive samples, will be referred for testing.

Collection Instructions

Citrate tubes must be completely filled in order to perform testing. The level of blood must be within the top and bottom borders of the black marker on the tubes.

Transport Instructions

Transport ambient at room temperature

Storage Instructions

Store refrigerated at 4°C

Testing Frequency

None

Min Test Volume

1mL

Add On Test Suitability

Room Temperature	4 hours
Refrigerated (2 - 4°C)	8 hours
Frozen Plasma* (-20°C)	up to 3 months**

*: Plasma must be double spun and frozen in line with LWI-COA-003.
**: Subject to sample availability

Container ID	C, CP, S, CH
CSR Instructions	All samples should be handled per standard procedures and forwarded to Haematology Central Laboratory. DO NOT REJECT SAMPLES. Instructions for: Heidelberg Specimen Reception Please place frozen aliquots into the Special Coag bucket in the -80°C core lab freezer
Laboratory Instructions	After centrifugation, coagulation specimens remain viable for up to 8 hours at room temperature (RT). Processing instructions for: Regional Laboratories Please ensure routine coagulation testing is validated prior to double centrifuging for Complex Haemostasis testing. Create a Platelet Poor Plasma aliquot by double centrifuging, aliquoting, and freezing (in line with LWI-COA-003). Refer to SOP-COA-002 for the number of aliquots required for this test. Storage instructions for: Regional Laboratories Store Platelet Poor Plasma aliquots frozen at -20°C. Store Primary Sodium Citrate refrigerated at 4°C. Transport instructions for: Regional Laboratories Transport Platelet Poor Plasma aliquots frozen in biofreeze bottles. Instructions for: Haematology - Central Laboratory Prepare aliquots and a copy of the request form for the Specimen Reception Sendaway team. All referalls must be accompanied by a completed 4T Score Form. This form is to be completed by laboratory haematology registrar.
External Laboratory	Haematology, Monash Health Pathology
Accredited Test	Yes