One single balloon Pytest Urea breath test should be collected 10 minutes after ingesting capsule.

Children and pregnant or breast-feeding women should consult their doctor before conducting this test.

Patients should consult with their doctor prior to ceasing any medications.

Prior to the test please ensure the patient follows these instructions:

• Exclusion Period	Medication Class	Common Types & Names
  4 weeks Prior	Antibiotics & Bismuth	- All antibiotics - Bismuth
  14 Days Prior	Sucralfate	- Carafate
  7 Days Prior	Proton Pump Inhibitors	- Losec (Omeprazole) - Somac (Pantaprazole) - Zoton (Lansoprazole) - Nexium (Esomeprazole) - Pariet
  During Fasting Period	Antacids & H2 Antagonists	- Aluminum hydroxide gel - Calcium carbonate (Alka-Seltzer, Tums) - Magnesium hydroxide (Milk of Magnesia) - Gaviscon, Gelusil, Maalox, Mylanta, Rolaids. - Pepto-Bismol, Zantac, Tarzac, Rani 2, Ranitidine, Eno,      Dexal, Gastrogel

Note: Patients must wait approx. 6-8 weeks after finishing a course of antibiotics to check for Helicobacter Pylori Eradication

Collection Procedure

1. Ensure patient is fasting, including no water or smoking, 6 hours before test and meets test criteria regarding medications
2. Place Helicobacter Pylori Breath Test Questionnaire sticker onto referral and complete questions
3. Remove contents from Box
4. Fill both cups with 30 mls of water each
5. Set timer for 10 minutes
6. Ask Patient to swallow capsule with 1^st cup of water and start the timer
7. After 3 minutes ask Patient to swallow 2^nd cup of water
8. Insert straw into the neck of the balloon and ensure all patient details are on the balloon
9. Assemble white transport box
10. When timer reaches 10 minutes, ask patient to take a deep breath and hold for 10 seconds.
11. Patient is to blow steadily into straw until balloon is firm
12. Grasp neck of balloon and twist removing straw and tie a knot into the end of the balloon
13. Place balloon into transport box labelled with the patient details
14. Put request into a specimen bag and attach to outside of white transport box with tape
15. Send into lab with courier

Negative