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Helicobacter Pylori Breath Test - C14

Alternate Names
Urea Breath Test
Ordering Information

One single balloon Pytest Urea breath test should be collected 10 minutes after ingesting capsule.

Children and pregnant or breast-feeding women should consult their doctor before conducting this test.

Patients should consult with their doctor prior to ceasing any medications.

Prior to the test please ensure the patient follows these instructions:


  • Exclusion Period


    ·         Common Types

    4 weeks Prior

    Antibiotics & Bismuth


    All Antibiotics, Bismuth


    14 Days Prior





    7 Days Prior 

    Proton Pump Inhibitors

    Losec (Omeprazole), Somac (Pantaprazole)

    Zoton (Lansoprazole), Nexium (Esomeprazole)


    During Fasting Period

    Antacids & H2 Antagonists

    Aluminum hydroxide gel.

    Calcium carbonate (Alka-Seltzer, Tums)

    Magnesium hydroxide (Milk of Magnesia)

    Gaviscon, Gelusil, Maalox, Mylanta, Rolaids.

    Pepto-Bismol, Zantac, Tarzac, Rani 2, Ranitidine, Eno, Dexal, Gastrogel


Note: Patients must wait approx. 6-8 weeks after finishing a course of antibiotics to check for Helicobacter Pylori Eradication

Referred Test
See collection instructions
See collection instructions
Additional Collection Instructions

Collection Procedure

  1. Ensure patient is fasting, including no water or smoking, 6 hours before test and meets test criteria regarding medications
  2. Place Helicobacter Pylori Breath Test Questionnaire sticker onto referral and complete questions
  3. Remove contents from Box
  4. Fill both cups with 30 mls of water each
  5. Set timer for 10 minutes
  6. Ask Patient to swallow capsule with 1st cup of water and start the timer
  7. After 3 minutes ask Patient to swallow 2nd cup of water
  8. Insert straw into the neck of the balloon and ensure all patient details are on the balloon
  9. Assemble white transport box
  10. When timer reaches 10 minutes, ask patient to take a deep breath and hold for 10 seconds.
  11. Patient is to blow steadily into straw until balloon is firm
  12. Grasp neck of balloon and twist removing straw and tie a knot into the end of the balloon
  13. Place balloon into transport box labelled with the patient details
  14. Put request into a specimen bag and attach to outside of white transport box with tape
  15. Send into lab with courier


Minimum Adult Volume
Minimum Paediatric Volume
Once a week
Reference Interval
Test Code
Storage Instructions
Store specimen at Room Temperature
Transport Instructions
Transport at room temperature
External Laboratory
Biochemistry, St Vincent's Hospital Pathology, Melbourne